Separation science will be explored by building on a survey of fundamental physical principles to understand processes of extraction, and technologies such as solid phase microextraction, supercritical fluid extraction, immunoaffinity extraction and molecularly imprinted polymers. Plate and rate theory will be developed to consider various forms of gas and liquid chromatographic methods, including hyphenated techniques that bridge to information detectors such as mass spectrometers. New opportunities for chromatography and separations by movement to small scale size will be considered by focusing on microfluidics, electro-osmotic flow and chip based microdevice applications. Applications examples will focus on problems in life sciences, forensics and environmental chemistry. Course work will include independent literature reviews and student presentations.